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Ovulation Midstream
INTRODUCTION
One Step LH Urine Ovulation Test is fast and easy-touse. It is a qualitative test that can predict when there is a LH (Luteinizing Hormone) surge, and in turn when you are likely to ovulate.

WHEN TO BEGIN TESTING
First, you must determine the length of your menstrual cycle. This is the number of days from the first day of your menstrual bleeding to the day before your next bleeding begins. Please refer to the chart to determine when you should start testing. If your cycle is shorter than 21 days or longer than 38 days, consult your doctor. If you do not know your cycle length, you may begin the test 11 days after your first period since the average cycle length is 28 days. Perform 1 test each day over a 5 day period, or until the LH surge has been detected.
URINE COLLECTION
1. Do not use first morning urine samples as LH is synthesized in your body early in the morning. It will not show up in your urine until later in the day.
2. The best time to collect your urine is between 10am - 8pm.
3. Collect urine at about the same time each day.
4. Reduce liquid intake about 2 hours before collecting your urine as a diluted urine sample can prevent the test from detecting LH surge.

TEST PROCEDURE

1. Allow the sealed LH Midstream test pouch, patient’s urine sample and control solution to reach room temperature (18- 30°C).

2. Remove the LH Midstream test device from the sealed pouch and use it as soon as possible.

3. Hold the handle of the test with one hand. Use the other hand to remove the cap and expose the absorbent sampler. Put the cap aside for now.

4. Point the absorbent tip downward, place the absorbent tip in urine stream for at least 10 seconds to be thoroughly wet. Otherwise, you can collect your urine into a clean cup and dip half of the absorbent pad into the urine for at least 5 seconds.

5. Re-cap the device and wait for color bands to appear. Depending on the concentration of LH, positive result may be observed in as short as 40 seconds. However, to confirm negative results, the complete reaction time (10 minutes) is required. It is important that the background is clear before the result is read. Do not read results after 30 minutes.


INTERPRETATION OF RESULTS
No LH Surge: Only one color band appears on the control (C) region or the test (T) band is lighter than the control band. There is no LH surge. LH Surge: If two color bands are visible and the test (T) band is equal to or darker than the control (C) band, one will probably ovulate in the next 24-48 hours. If one wants to be pregnant, the best time to have intercourse is after 24 but before 48 hours. Invalid: No visible band at all or no colored band appears on the control (C) region. Repeat test with a new test kit. If the problem persists, discontinue using the test kit immediately and contact your local distributor.

STORAGE AND STABILITY
The test kit can be stored at temperatures between 2 to 30°C in the sealed pouch to the date of expiration. The test kit should be kept away from direct sunlight, moisture and heat.

PRECAUTION
1. For in vitro diagnostic use only.
2. Do not use test kit beyond expiry date.
3. The test device should not be reused.

LIMITATIONS
1. As with all diagnostic tests, all results must be considered with other clinical information available to the physician. A definite clinical diagnosis should only be made by the physician after all clinical and laboratory findings have been evaluated.

2. The test results should not be affected by pain relievers, antibiotics and other common drugs. After using the pill or patch that contains hCG or LH may affect the test result and should not be taken while using this test kit. In addition, the test will not work properly if you are pregnant or menopausal.

3. A positive result means your urine contains the level of LH that would indicate you are beginning to ovulate. You can assume that you will ovulate within 24-36 hours. However, not every woman ovulates mid-cycle; therefore, you may not see a positive result during the first 5 days of testing.
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