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Test Instructions |
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| Pregnancy Midstream |
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INTRODUCTION
One Step Midstream Style HCG Urine Pregnancy Test is a
test kit for the determination of hCG (Human Chorionic
Gonadotropin) in urine specimens. There is an
appearance of hCG in both urine and serum soon after
conception, and its subsequent rapid rise in concentration
during early gestational growth. This test is used to obtain
a visual, qualitative result for the early detection of
pregnancy.
TEST PROCEDURE
1. To begin testing, open the sealed pouch by tearing
along the notch. Remove the test kit from the pouch
and use it as soon as possible.
2. Hold the handle of the test with one hand. Use the
other hand to remove the cap and expose the
absorbent. Put the cap aside for now.
3. Point the absorbent tip downward; place the absorbent
tip in urine stream for at least 10 seconds to be
thoroughly wet. Otherwise, you can collect your urine
into a clean cup and dip half of the absorbent pad into
the urine for at least 10 seconds.
4. Re-cap the device and wait for colored bands to
appear. Depending on the concentration of hCG,
positive results may be observed in as soon as 40
seconds. However, to confirm negative results, the
complete reaction time of 5 minutes is required. It is
important that the background is clear before the result
is read. Do not read results after more than 30
minutes.
PRECAUTION
1. For in vitro diagnostic use only.
2. Do not use test kit beyond expiry date.
3. The test device should not be reused.
STORAGE AND STABILITY
The test kit can be stored at temperatures between 2 to
30°C in the sealed pouch to the date of expiration. The
test kit should be kept away from direct sunlight,
moisture and heat.
INTERPRETATION OF RESULTS Negative: Only one color band
appears on the control region. No
apparent band on the test region.
This indicates that no pregnancy has been detected.
Positive: Distinct color bands
appear on both the control and test
regions. Presence of both test line
and control line indicate that you
are probably pregnant. The color intensity of the test bands
may vary since different stages of pregnancy have different
concentrations of hCG hormone.
Invalid: No visible band at all or no band appears on the
control (C) region. Repeat with a new test kit. If the problem
persists, discontinue using the test kit immediately and
contact your local distributor.
LIMITATIONS
1. Very dilute urine specimens, as indicated by a low specific
gravity, may not contain representative levels of hCG. If
pregnancy is still suspected, a first morning urine specimen
should be collected 48 hours later and tested.
2. False negative results may occur when the levels of hCG
are below the sensitivity level of the test. When pregnancy
is still suspected, a first morning urine specimen should be
collected 48 hours later and tested.
3. Very low levels of hCG (less than 50 mIU/mL) are present
in urine specimen shortly after implantation. However,
because a significant number of first trimester pregnancies
terminate for natural reasons (5), a test result that is weakly
positive should be confirmed by retesting with a first
morning urine specimen collected 48 hours later.
4. A number of conditions other than pregnancy, including
trophoblastic disease and certain non-trophoblastic
neoplasms including testicular tumors, prostate cancer,
breast cancer, and lung cancer, cause elevated levels of
hCG (6-7). Therefore, the presence of hCG in urine should
not be used to diagnose pregnancy unless these conditions
have been ruled out.
5. This test provides a presumptive diagnosis for pregnancy. A
confirmed pregnancy diagnosis should only be made by a
physician after all clinical and laboratory findings have been
evaluated.
NOTES
The appearance of a red line in the control region (C)
tells you that you followed the test procedure properly
and the proper amount of urine was absorbed.
You can test your urine as early as the first day you
miss your period. You can perform the test anytime of
the day; however, if you are pregnant, first morning
urine contains the most pregnancy hormone.
Drugs which contain hCG or LH can give a false positive
result. Alcohol, oral contraceptives, painkillers,
antibiotics or hormone therapies that do not contain
hCG should not affect the test result. |
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